Thermo scientific genejet plasmid miniprep kit for 50 preps thermo scientific genejet plasmid miniprep kit dna extraction and purification. Qiaprep 8 miniprep kit 28 qiaprep 8 turbo miniprep kit 31 qiaprep 96 turbo miniprep kit 33 cell cultivation in a 96well block 36 special applications 37 troubleshooting guide 38 appendix a. Efficienthigh yields of up to 20 g of high quality plasmid dna. By combining silicabinding technology and the convenience of a spin column format, up to 15. Highlights genejet plasmid miniprep kit has been tested in the isolation of puc19 dna from e. L of elution buffer to the column and incubate 2 minutes. Highlights the resuspension solution should be stored at 4c and is stable for 6 months. For longer storage, it is recommended to keep the kit at 4c. Genelute plasmid dna miniprep kit 350 purifications gen. C plasmid preparations with the genelute kit, comparing either the standard miniprep procedure or the miraprep using 1x volume of ethanol. Simply add the uniquely formulated 7x lysis buffer directly to.
Genejet plasmid miniprep kit 250 prep k0503 from thermo. Part of the zymopure plasmid kits collection, the zymopure plasmid miniprep kit features a spin columnbased method for the purification of up to 100 g of ultrapure endotoxinfree plasmid dna. Thermo scientific genejet plasmid miniprep kit utilizes an exclusive silica based membrane technology in the form of a convenient spin column. Genejet plasmid maxiprep kit largescale isolation of up to 750. Overnight bacterial culture with chassis containing plasmid of choice 1 5 ml plasmid purification kit i. Each genejet spin column can recover up to 20g of plasmid dna. The qiaprep spin miniprep kit is designed for isolation of up to 20.
Thermo scientific genejet plasmid maxiprep kit, sufficient for 25 preps, utilizes silicabased membrane technology in the form of convenient spin column. Thermo scientific genejet resuspension solution is a component of the genejet plasmid miniprep kit k0502k0503 and may be purchased separately. Laboratory chemicals details of the supplier of the safety data sheet manufacturersupplier. The quality of isolated dna has been evaluated in a set of tests. Solution i once rnase a is added should be stored at 28. Plasmid extraction from bacteria miniprep purification o f p lasmid d na from 1 5 m l e. The monarch plasmid miniprep kit is a rapid and reliable method for the purification of high quality plasmid dna. We provide a wide range of size formats from mini to gigapreps as well as options for highthroughput processing. If you want to perform plasmid purification without using a kit, you can find a protocol for kitfree plasmid mini prep at the bottom of this page. C plasmid preparations with the genelute kit, comparing either the standard miniprep procedure or.
The kit utilizes an exclusive silica based membrane technology in the form of a convenient spin column. This system can be used to isolate any plasmid from e. This kit is able to purify plasmids up to,000 bp in size, and the typical. Thermo scientific genejet plasmid maxiprep kit for 25. The zyppy plasmid miniprep kit may also be used with the classical centrifugebased procedure for processing up to 3 ml of bacterial culture. Agarose gel analysis of plasmid dna 45 references 45.
Each column has a binding capacity of at least 20 g. To help you choose which kit is the best choice for your application see our plasmid kit selection guide. Perform isolation of high quality plasmid dna from recombinant e. Please do not hesitate to contact us if you have any questions. Resuspension solution for genejet plasmid miniprep kit. Thermofisher genejet plasmid miniprep kit catalog no.
The protocol provides a simple and reliable method to achieve the rapid isolation of highly purified plasmid. All other materials should be stored at room temperature. Product information thermo scientific genejet plasmid. Genejet plasmid miniprep kit components of the kit. Zymopure ii plasmid kits get free sample now zymo research. The plasmid dna is preferentially purified from other cellular components such as genomic dna and rna. Genejet plasmid miniprep kit from fermentas biocompare. Genespin miniprep plasmid purification kit v2 for purification of highquality plasmid dna ver. Thermo scientific genejet plasmid miniprep kit fisher. Compare genejet plasmid miniprep kit 250 prep k0503 from thermo fisher scientific on. Genejet plasmid miniprep kit thermo fisher scientific. Each prep recovers up to 750 g of high copy plasmid dna that can be used in a wide variety of molecular biology procedures, such as restriction en. Please consult the material safety data sheet for information regarding hazards and safe handling practices.
The zyppy plasmid miniprep kit is a plasmid dna kit that features a pelletfree modified alkaline lysis system that bypasses the normal cellpelleting and resuspension steps common to classical plasmid preparation procedures. Thermo scientific genejet plasmid miniprep kit for 50. Isolation of plasmid dna from agrobacterium using the qiaprep spin miniprep kit. Genejet plasmid miniprep kit can be stored for up to 12 months at room temperature 1525c. Plasmid dna mini kit i and plasmid dna mini kit ii components are guaranteed for at least 12 months from the date of purchase when stored as follows. Genelute plasmid dna miniprep kit 350 purifications. The system provides a rapid method to purify plasmid dna using a silica membrane column. If you will be using a kit, follow the kits instructions. If precipitate forms in the buffers during storage, it should be redissolved by incubating the buffers at 37c before use. Important notes the yield of plasmid dna using the miniprep kit depends on. The genejet plasmid miniprep kit is designed for rapid and costeffective smallscale preparation of high quality plasmid dna from recombinant li cultures. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators.
Recovering plasmid dna from bacterial culture addgene. Description the kit utilizes silicabased membrane technology in the form of convenient spin column. High quality dna without rna contamination can be prepared within 1015 min. Thermo scientific genejet plasmid miniprep kit has been tested in the isolation of puc19 dna from e. The kit recovers up to 20 g of high copy plasmid dna per isolation procedure. Genejet plasmid miniprep kit utilizes an exclusive silicabased membrane technology in the form of a convenient spin column.
Solution ii must be tightly capped when not in use. The plasmid dna is isolated using the qiaprep spin miniprep kit hy protocol versus. Qiaprep spin miniprep kit highyield protocol english pdf. Thermo scientific genejet plasmid miniprep kit for 50 preps. All centrifugation steps should be carried out at 16,000 x g,000 rpm. Plasmid dna can be purified in less than 10 minutes, depending on the number of samples processed. Thermo scientific genejet plasmid miniprep kit life. Supplier page from thermo fisher scientific for genejet plasmid miniprep kit 250 prep product specs. The genejet plasmid miniprep kit is a simple, rapid, and costeffective system for the isolation of high quality plasmid dna from recombinant e. The kit is tested for the isolation of puc19 dna from li according to the protocol described. Simply add the uniquely formulated 7x lysis buffer directly to your bacterial culture, neutral.
General description the genelute plasmid miniprep kit offers a simple, rapid, and costeffective method for isolating plasmid dna from recombinant e. Plasmid miniprep kit this kit is designed for the rapid preparation of plasmid dna from small cultures of escherichia coli using convenient spin columns. Qiagen is the leading provider of innovative sample. Easypreptm plasmid miniprep 5 6 kit contents at 5,000 g for 10 min or 3,500 rpm for 20 min in a tabletop pellet by vortexing or pipetting. The monarch plasmid miniprep kit is a rapid and reliable method for the purification of high quality plasmid dna from bacterial cultures. Background information 41 growth of bacterial cultures 41 preparation of cell lysates 44 appendix b. The entire miniprep procedure can be completed in 30 minutes or less, depending on the number of samples processed.
Thermo scientific genejet plasmid miniprep kit utilizes an exclusive silicabased membrane technology in the form of a convenient spin column. The pureyield plasmid miniprep system isolates highquality plasmid dna for use in eukaryotic transfection and in vitro expression experiments. The axyprep plasmid miniprep kit is based upon a modified sdsalkaline lysis of bacterial cells in combination with selective binding of the plasmid dna to a special miniprep column. If you plan to perform transfections and prefer a miniprep format try our nucleospin plasmid transfectiongrade kits. This method employs standard cell resuspension, alkaline lysis, and neutralization steps, with the additional benefit of color indicators at certain steps to easily monitor completion. After the addition of rnase a, the resuspension solution is stable. The genelute plasmid miniprep kit offers a simple, rapid, and costeffective method for isolating plasmid dna from recombinant e. By combining silicabinding technology and the convenience of a spin column format, up to 15 g of high copy plasmid dna can be recovered from.
This kit is designed for the rapid preparation of plasmid dna from small cultures of escherichia coli using convenient spin columns. Plasmid dnas purified from this kit can be used directly for downstream applications such as transfection, cloning, sequencing, as well as in vivo applications. The protocol below is meant to describe the general procedure for purifying plasmid dna from bacterial cultures. The quicklyse miniprep kit provides a rapid spincolumnbased method for isolating sequencinggrade plasmid dna. Learn how to purify molecular grade plasmid dna in 15 minutes with the thermo scientific genejet plasmid miniprep kit. Thermo scientific genejet plasmid miniprep kit promotion. The kit utilizes an exclusive silicabased membrane technology in the form of a convenient spin column. Highlights efficienthigh yields of up to 20 g of high quality plasmid dna fastpr. Genejet plasmid miniprep kit 250 prep from thermo fisher scientific description the genejet plasmid miniprep kit is a simple, rapid, and costeffective system for the isolation of high quality plasmid dna from recombinant e.
Genejet plasmid miniprep kit k0502, k0503 wash the column add 500. The yield of the highcopy plasmid from this kit is about 1020g. Thermo scientific genejet plasmid miniprep kit thermo fisher. Sequencing chromatogram of plasmid dna pgem purified using the zyppy plasmid miniprep kit.
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